Mycobacterium tuberculosis complex Antimicrobial Susceptibility Testing (Culture, 2nd Line Drugs)

Test Order Name - Mycobacterium tuberculosis Complex Drug Susceptibility Testing, Second Line (Referred Testing to MDL-CDPH)

Synonym(s) - Antimycobacterial susceptibility testing to aid in the treatment of patients with tuberculosis (2nd line drugs).

Methodology - The BACTEC MGIT 960 is used as a rapid qualitative procedure for susceptibility testing of M.tb. complex from culture. Drugs tested are amikacin 1.5 microgram per milliliter, moxifloxacin 0.25 microgram per milliliter, capreomycin 3 microgram per milliliter, ethionamide 5 microgram per milliliter.

Acceptable Specimen Type(s) for Testing - Pure culture isolates of M.tb. complex from any specimen type/source.

Transport / Collection Medium - Pure cultures on Lowenstein-Jensen Slants, BACTEC MGIT Liquid Medium, Middlebrook 7H9 Broth

Storage and Preservation of Specimen - Freshly grown culture of M.tb. complex: no more than 14 days from inoculation for solid culture and no more than 5 days from inoculation for BACTEC MGIT liquid media. Keep culture at ambient temperature to preserve if over the recommended age of culture.

Minimum Volume Required - 4 milliliters of BBL Middlebrook 7H9 or MGIT medium, or solid culture

Additional Collection Instructions - Fresh, pure growth of M.tb. complex is required for this test. Identification of M.tb. complex must be confirmed before testing.

Additional Required Information - Collection date and specimen source required. Indicate first line drug susceptibility results and patient history. Prior approval with the Microbial Diseases Laboratory (MDL) required.

Send Out? - Yes

Turnaround Time - 7 to 14 days

Testing Restrictions - None

Limitations / Notes / Disclaimers - Results may be unobtainable due to cultures mixed with Non-tuberculous Mycobacteria.