Escherichia coli (Shiga Toxin Producing) Serogrouping (including O157)

Test Order - Shiga Toxin Producing Escherichia coli (STEC) Serogrouping and Toxin Testing (including E. coli O157)

Synonym(s) - E. coli O157 serogrouping and toxin testing, non-O157 STEC serogrouping and toxin testing

Methodology - Culture, biochemical identification, latex agglutination, real time Shiga toxin gene (stx1/stx2) PCR, immunochromatographic rapid toxin assay

Acceptable Specimen Type(s) - for Testing Pure isolates, previously identified as Escherichia coli

Transport / Collection Medium Transport - on nutrient agar slant, packaged and sealed according to regulations.

Storage and Preservation of Specimen - Refrigerate until transported to the laboratory. Transport at room temperature.

Minimum Volume Required - None

Additional Collection Instructions - None

Additional Required Information - Please include identification results with submission.

Send Out? - Yes (Shiga toxin gene PCR and limited serogrouping performed in-house)

Turnaround Time - Up to 8 weeks

Testing Restrictions - None

Limitations / Notes / Disclaimers - This Shiga toxin gene PCR assay was developed, and its performance characteristics were determined by the San Francisco Public Health Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such approval is not necessary and results should not be regarded as investigational or for research.