Test Order Name - Lymphogranuloma venereum (LGV) Real-time PCR
Synonym(s) - LGV PCR
Methodology - Real-time polymerase chain reaction (PCR)
Acceptable Specimen Type(s) for Testing - Rectal and genital swabs.
Transport / Collection Medium -
- Universal (or viral) transport medium (UTM or VTM): urethral, rectal and vaginal specimens. If Chlamydia testing was not performed prior to submission please submit an Aptima or Alinity m swab from the same specimen source as the UTM/VTM swab.
- Aptima® Unisex Swab Specimen Collection Kit (catalog # 301041): Urethral specimens only
- Aptima® Multitest Swab Specimen Collection Kit (catalog # PRD-03546): for rectal and vaginal specimens. Alinity m multi-collect specimen collection kit: rectal and vaginal specimens.
- If either Aptima® Unisex or Multitest, Alinity multi-collect Collection kit or UTM/VTM is submitted: Please indicate whether Chlamydia NAAT screening has already been performed on specimen and date of detection. If requesting CT screening in addition to LGV PCR, please indicate both tests on requisition.
Storage and Preservation of Specimen -
UTM or VTM: Store at 2 to 8⁰C for up to 72 hours; otherwise freeze at or below -30⁰C.
APTIMA collection kits: Store at room temperature for up to 60 days.
Alinity m Multi-collect kits: specimens: Store at 2 to 30°C for 14 days and at 25°C to -15°C for longer storage. (Avoid more than 4 freeze though cycles).
Minimum Volume Required - 2 milliliters Aptima Collection kits 500 microliters Alinity m Multi-collect collection kits.